Shigella (Shigellas dysenteriae)

Technical details for ready to use PCR kit Shigella (Shigella dysenteriae) (gel agarose based)

K741 Shigella (Shigellas dysenteriae), 100 reactions

Shigella, the causative agent of shigellosis or "bacillary dysentery," has been increasingly involved in foodborne outbreaks. Shigella was the third most reported foodborne bacterial pathogen in 2002. Foods are most commonly contaminated with Shigella by an infected food handler who practices poor personal hygiene. Shigella is acid resistant, salt tolerant, and can survive at infective levels in many types of foods such as fruits and vegetables, low pH foods, prepared foods, and foods held in modified atmosphere or vacuum packaging. Survival is often increased when food is held at refrigerated temperatures. Detection methods for Shigella include conventional culture methods, immunological methods, and molecular microbiological methods. Conventional culture of Shigella in foods is often problematic due to the lack of appropriate selective media. Immunological methods for Shigella have been researched. The PCR is the method of choice for detection of Shigella. Usually it is the Shigella dysenteriae, which causes disease. Therefore identification of this is very important.

Targetgene: highly conserved gene region of Shigella (dysenteriae).

Kinds of probes: direct detection in tissue, water, stool, blood and other body tissues.

One can isolate the DNA from the sample through commercial available kits or in house methods.

Validation: checked for cross reactions with related and nonrelated micro organisms like Salmonella, Yersinia enterocolitica, Legionella, Campylobacter jejuni, Staphylococcus and many more.

Detection limit: one bacterium in sample

Contents of the kits: Nucleotides, polymerase, buffers, PCR master mix, loading dye, positive control, negative control as well as molecular marker along with the manual.

They are ready to use form as there are only 2 steps to do for Thermocycling.

The detection can be done in gel agarose test.

- Gene sequencing is optional
- internal control is optional

This kit is only for in vitro use. This is only for veterinary use. For human use, only for research use. To be used through technical person