genekam logo
  • usa
    • german
    • russia
    • china
Duissern Str. 65a · 47058 Duisburg · Germany
Tel. +49 203 / 555858-31,-32,-33 · Fax: +49 203 / 35 82 99
menuicon
slider
Latest News:

08.09.2017
Genekam has developed human antibodies (true antibodies, therapeutic antibodies) against H5N1 and now we are working to generate sufficient quantity to conduct other assays!

Latest News:

16.08.2017
Genekam has formed a new company called
3D Genome Medicine Inc. in order to start operations in USA. Shortly website and adress!

  • BIO 2018

    Visit us at BIO 2018 in Boston, USA:
    04.06. - 07.06 2018

  • MEDICA 2017

    Visit us at MEDICA in Duesseldorf, Germany:
    13.11.17 - 16.11.17

  • BIO 2018

    Visit us at BIO 2018 in Boston, USA:
    04.06. - 07.06 2018

  • MEDICA 2017

    Visit us at MEDICA in Duesseldorf, Germany:
    13.11.17 - 16.11.17

Neural differentiation media (10X)

Cat.
No.
Name of ready to use
PCR Kit
Storage DOC Amount Price*
STEM07Neural differentiation media (10X) -20°C 10ml150,-€

Neural cell differentition of MSC (Steril Product)*


Kit contents: STEM07 Neural cell differentition of MSC: 10X = 10 ml (store at -20°C)
Final Neural cell differention media: Add 10 ml of Genekam neural cell differentiation media into 90 ml of DMEM + 15% FCS + 1% SP + 1% Glutamine or in your culturing media. Store it at 4°C. You can prepare less media, but the ratio should be 1:10.

After you have stem cells e.g. MSC. To isolate MSC cells, one can use Genekam Stem cell isolator kit. After you get 60-85% confluence during culturing of stem cells, please remove the cell using trypsin or Genekam cell remover and wash the cells to make free from trypsin while Centifuge at 1400 rpm for 5 minutes and suspend the pellet now to make the cell count using trypan blue.
MSC were seeded on glass coverslips coated with 1 mg/ml p-lysine and 13 ug/ml laminin in final neural cell differentiationmedia for 4 weeks with feeding interval of 3-5 days with final media. One can do immunostaining against gamma amino butyric acid (GABA) and tyrosine-hydroxylase (TB).

* only for research use.
TOP