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16.08.2017
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07.07.2017
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Circulating Tumour Cells*

Isolation of circulating tumour cell kit with two markers and PCR Kit for Biomarkers of Melanoma from CTC


Human circulating tumour cells (CTC) in blood are an important indicator of the progression of a cancer and in some cases, these may be an indication of so called sleeping tumour cells i.e. it does mean that there is an active disease. Therefore PCR tests are being conducted in many laboratories around the world for presence of markers of such blood cells. As the number of such cell is very low, therefore there is a need of enrichment of these cells before doing RNA isolation. Genekam Biotechnology AG has developed this enrichment step and this can be combined with mini-column method or other method. Genekam offers this enrichment step as sterilised product because some users may want to grow enriched cells in order to do other studies including nucleic cells isolation e.g. tumour resistance (if possible!), but that one can go directly to RNA isolation step after enrichment even under unsterilised environment in order to run the tumour marker PCRassay. There is only 1 circulating tumour cell in one million of mononuclear cells, therefore enrichment is very important step during the conducting PCR for human cancer markers from blood. Peripheral blood is one of easiest way to access against the biopsy of lymphnodes or bone marrow, hence it is an excellent idea, if one can develop more and more assays based on CTC.

Important feature of enrichment step is that one can do the isolation of circulating tumour cells directly from blood 1ml or 3ml and there is no need of using density gradient method.

Genekam CTC Enrichment kit is very simple to use: There are only 3 steps:

  1. isolation of all cells from 1 or 2 ml of blood to develop a pellet
  2. adding of the beads to this pellet
  3. Isolation of specific cells - now you can use them for mini-column isolation or your in-house nucleic acid isolation

 

Cat.-No. Name Amount Price
STEM21 Genekam circulating tumour cells (CTC) enrichment kit 15 139,- €
STEM22 Genekam circulating tumour cells (CTC) enrichment kit 30 249,- €

This product can be used only with human blood.

We are developing isolation of circulating tumour cell with two different markers as one marker is not sufficient. In coming days, we are going to put a number of pictures of this method on our website along with the cells grown in cell cultures to conduct further studies.

Cat.-No. Name Amount Price
K418 PCR kit for Melanoma Biomarker (Tyrosinase) 100 699,- €

Other Biomarkers like MART, MAGE and others are under development. The results can be seen on the gel agarose. It means that this method can be used under the field conditions, where one has access to conventional PCR machine, which is available as cheap as US $ 700 dollar.

This product is developed and validated with human melanoma samples.

* for research use only

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