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Endotoxin removal kit

Genekam endotoxin removal Kit (to remove endotoxin): There are many applications, where endooxins are causing serious problems. Many groups from institutes, universities and biotech industry are working on recombinant proteins expressed in bacteria like E. coli and othe organisms, hence the isolated proteins from much cultures contain a lot of lipopolysaccharides (LPS) so called endotoxins, which can alter the results of isolated proteins during their applications, therefore there is an urgent need to remove them before applying these proteins as therapeutics, diagnostics reagents (medical devices), antibodies (humanised / other origin), vaccines etc. and it is highly recommended to remove them from recombinant proteins in order to start in vitro, preclinical as well as clinical studies. The presence of endotoxins in injectable compounds may lead to sepsis (septic shock!) resulting in the failure of a good compound. Endotoxins may lead to serious problems during the cell cultures. The source of endotoxin may be water, components as well as sera to be used during the culturing or fermentation process. There are different methods available to remove them so e.g. ultracentrifugation, ultrafiltration, chemical treatment etc. None of the methods is going to remove the endotoxin fully, hence a combination of these methods may be alternative. Chemical treatment of isolated protein may leave traces of the chemical leading to side effects during the therapy or development of therapies. Genekam has developed magnetic beads to remove them from isolated proteins. The method is very simple as one can add magnetic beads to the solution, keeps it for 30 minutes and remove the beads (on which the endoxins bind) with a magnet or magnetic field. Genekam beads are designed in such a way that they will remove the maximum endotoxins in the first attempt. However it may be possible that one has to repeat it to remove the endotoxins depending upon the grade of contamination. Genekam beads can be used to remove the LPS from cell cultures, liquids (including water) etc. Even these beads can be used to remove endotoxins from nucleic acid solutions. At present, Genekam offers to remove the endotoxins from small volume samples. If the user has a need to remove endotoxins from large scale samples, Genekam offer here solutions for large scale user.There are many companies around the world, which are producing recombinant proteins or other products for cell therapies, advanced tissue therapies etc. One should test the products for the presence of endotoxins before applying them for different uses. Even the cell culture media should be tested to meet the lowest possible endotoxin concentrations. Technology of Genekam is of great help. Genekam endotoxin removal kit is a sterile product, hence it can be used in sterile applications like cell cultures and sterile proteins (to be used for in vivo research applications). One can regenerate used magnetic beads to be used again. There are many products for which there are legal requirements in EU or USA from FDA, which must be met. During the fermentation process, one applies a number of bioreactors, hence the surface of reactors may be contaminated with biofilms containing endotoxins, we think that one can use Genekam magnetic beads to reduce the load of endotoxins after chemical or mechanical treatment of these reactors. Even Genekam magnetic beads may be the part of the mechanical treatment of bioreactors. These beads can be used remove with magnetic field or with water from bioreactors. Glasswares may be one of major source of the endotoxins contamination as endotoxins binds on glass surface very strongly. Heating does not remove the endotoxin fully, therefore Genekam magnetic beads should tried to remove them too. Tip for biopharmaceutical industry: Genekam carries huge number of tests for detecting the pathogens during the fermentation process and Genekam has developed a number of products e.g. Mycoplasma removal. Please try to use them.

Properties:
  • Fast
  • Simple
  • Maximum removal of the endotoxin in first attempt
  • Removal endotoxin from different sources
  • Sterile
  • Multiple use of the magnetic beads possible leading to reduce in cost
  • To remove the endotoxins from protein as well as nucleic acid solutions
Magnetic beads developed special to remove the endotoxins with special methods and chemicals