Human Herpes Simplex Virus 1

Technical details for realtime PCR kit Human herpes simplex virus

FR018 Human Herpes Simplex Virus 1, 100 reactions

Standard laboratory technique for herpes detection is cell culture as one recognise the virus through cytopathic affects as well as later on serological detection. HSV 1 and 2 infections overlap in many clinical samples and these viruses have been isolated from skin, mucosa and visceral organs. Many studies have shown that PCR is the reliable method to detect the herpes viruses as well as other viruses. Moreover PCR is the fastest method as the results with the real time PCR can be finished within 3-4 hours. Moreover one can see the load of the virus in real time PCR and this can be monitored during the preventative measurements.

Target gene: highly conserved region of g region of HSV 1 and 2

Kind of probes: body fluids like saliva, blood (serum), tissue biopsies, nerve tissues, genital biopsies. This kit needs DNA, hence it should be isolated with good quality commercial kit or home made method. Genekam Biotechnology AG can ship you the 3rd party DNA isolation kit.

Key points: The kit has been checked for cross reactions with different herpes viruses like EBV, CMV and of animal origin like Equine Herpes viruses, Koi´s Herpes virus etc. and with non related micro organisms. They have been used for the different dilutions and for the sensitivity.

Detection limit: 10-50 genome copies of HSV1 and HSV2

Components of the kit:

Contents of Kit: primern, fluorogenic primers (filter FAM to used in one step PCR and filters FAM and VIC are to be used in 2 step PCR), nucleotides, buffers, molecular water, negative control, positive control and manual. Dyes used are 6-carboxy-fluorscein, YYE and 6-carboxytetramethylrhodamine.

Storage: -20 degree

- only for in vitro use
- only for research use
- to be used through technical persons