All our PCR Kits contain primers, +ve control, negative control, nucleotide, buffers, loading dye and molecular marker (in case of RNA kit: it contains RNA to cDNA chemicals reverse-transcriptase, ribonuclease inhibitor, dNTP etc.). You have to add your DNA or RNA template to them. Our kits can be used with any good-quality thermocycler availale on the market. Our kits expire after 1 year, if they are stored at -20°C. All of our products are made in our laboratory in Germany. We can give you the certificate of German origin, as some countries may need this certificate.