Genekam magnetic bead application in medicine*

Genekam Biotechnology AG has started developing different magnetic beads applications for cell culture, RNA / DNA isolation, protein isolations, transfection etc in 2007. At the beginning, Genekam carries only isolation kits. Today 2019, the range of products has increased to cover different field like protein purification, cell separation, next generation genesequencing, endotoxin removal and many other applications. Genekam is working to reduce the cost and increase the output with magnetic beads applications. Genekam is working on development more applications of magnetic beads, which are quite innovative and not known in the literature today to solve the complex problems of virology and immunoogy. We are developing our hardware solutions e.g. magnetic separators. Some of the pictures of these magnetic separators are already posted on Genekam website. The magnetic beads solutions can be next generation applications to work in automatic machine. Those, who are thinking to use magnetic applications, must keep in mind that magnets in Laboratory need precautions also as many companies are selling magnetic separator without any warning on them e.g. if you will touch the magnetic separator with mobile tablet or telephone, LCD may be destroyed and this can happen during taking the photo of such separators, if you are too close to magnet field with your device. Magnetic separators may interfere with the work of other instruments. Therefore it is better to make a special area in the laboratory, where one can work with magnetic separators and particles. One can visit our website to find new range of solutions. Most of kits are ready to use i.e. user can start with isolation of protein or cells immediately on arrival. Isolation of cells from blood will give more output than usual methods being used in standard laboratory. Genekam kits may help to enhence the productivity of development and manufacturing process of new therapeutic options like CAR T-cell therapies, human monoclonal antibodies, drug molecules and vaccines.

These can be used to isolate the DNA or RNA from the different sources like tissue, buccal swabs, plasma and blood. Shortly we are introducing the kits for plant DNA/RNA isolation.

This can be used on blood, bone marrow and other fluid samples

This can be used on blood, bone marrow and other fluid samples.

Magnetic cell separation

Magnetic cell separation from blood: In blood, there are many cells, which can be isolated to conduct various experiments like developing new kinds of assays and cell therapies. Separation of cells can be also done through magnetic field. This method is quite simple and can be conducted in laboratories without the help of complex and expensive instruments. Therefore Genekam Biotechnology AG has developed a number of solutions to do. These are different markers being expressed on the cells, which can be used as source of isolation in order to get pure population. After the isolation, one should do various experiments like microscopic and flowcytometry examination to be sure that the quality of cells is sufficient good to go ahead with further work. The offered solutions are sterile, hence isolated cells under sterile conditions can be used for cell cultures. These isolated cells can be used for molecular and immunological analysis. This kit can be used to isolate cells from as little as 1 ml of blood.

Isolation kit includes: magnetic beads, Fc-Receptor blocker, enrichment solution of white blood cells
The kit contains also components for enrichment of white blood cells, hence one can save time and money for density gradient method. However one can also use density gradient method, if it is essential to isolate the cells.

Optional (to be bought separately): Mac Buffer, magnetic separator

Procedure:

1. Enrichment of white blood cells
2. Magnetic separation of cells
3. Washing and collection

All kits are sterile products. If the user will work under sterile conditions, there will be isolated cells as sterile product to be used for cell culture and future applications.

This kit is for magnetic bead separation of dead and live cells under laboratory conditions. The method is very simple. Add Genekam magnetic beads to your cells, wait for 10-20 minutes and separate the cells with magnetic field. Genekam beads with attach to apoptotic cells. The product is sterile. After that user can process the cells for further applications. This product can be used on different kinds of cells including stem cells and sperms.

Transfection with magnetic beads

Fast +++ Simple ++ Many fold highter transfection rate than conventional system ++ Single as well as Multiple genes with one transfection +++ Easy Transfection of hard to transfect cells like Monocytes +++

Gene delivery, the process of introducing foreign DNA into host cells, is an important step in the genetic modification and is the basis of modern therapies like CAR T cell genetherapies, vaccines and development of recombinant proteins The key point in the success of this genetic transformation has been the development of a safe, stable and efficient gene delivery system. Over the past few decades, many different gene delivery methods have been developed, which can be divided into viral and non-viral types vector systems. Virus-mediated gene delivery utilizes the ability of a virus to inject its DNA into a host cell. Although viral carriers can achieve relatively high levels of transfection efficiency, they have still from many drawbacks, including potential risks of toxicity or immunogenicity, difficulty of production scale-up, and limited capacity to carry DNA beyond a certain size. Therefore, nonviral carriers like Genekam magnetic beads, which have several advantages such as minimal host immune response, stability in storage, and relative ease of production and scale-up, are being developed as alternative methods of gene delivery. Non-viral approaches include agrobacterium-mediated methods, chemical methods such as lipofection, and physical methods such as microinjection, gene guns, hydrostatic pressure, electroporation, continuous infusion, and sonication. Alongside developments in nanotechnology and molecular biology, nanoparticles with positively charged surfaces have shown promise as non-viral carriers for gene delivery. Superparamagnetic nanoparticles like Genekam magnetic beads have many advantages over other

-non-viral gene delivery systems

-enhanced resistance to digestion

-higher DNA carrying capacity

-more powerful penetration

-lower cost

-the ability to drive stable and efficient expression of target genes when exposed to an external magnetic field..

-single as well as multiple genes can be delivered

-simple to perform

-high rate of transfection

Magnetofection with Genekam magnetic beads is a simple, versatile and highly efficient method of gene transfer that uses magnetic force to promote the uptake of gene vectors associated with cationic magnetic nanoparticles into target cells. Effective gene delivery into the mammalian somatic cell nucleus is a key step in achieving a reproductive single cell clone and enabling the breeding of new varieties of animals using the somatic cell nuclear transfer technique. The application of magnetic nanoparticles as gene carriers has progressed rapidly in many fields and number of publications are on increase.

This system can be used for different plasmids, vectors like adeno, retro and orher virus vectors, antisense as well as PCR products. It can be used on different types of cells like monocytes, CHO, HELA, BHK, Vero, endothelial cells, lymphocytes, fibroblasts, chondrocytes, epithelial cells, renal cells, hepatocytes and many more.

Points to be kept in mind for in vitro magnetofection:

The non-adherent cells are to be centrifuged and one can add 30 ul of magnetic neads DNA complex to the pellet and let it mix for 30 minutes. Pipette them in plate or flask, where the magnetic field will be applied. One can also use coated plates so that these cells adhere to the surface. This is based on experience!

The adherent cells are to be grown upto 60-80% confluency at the time of transfection.

The cells between 2000 to 20000 per ml are needed for transfection. Vector is to be prepared in serum free media and add to the cells. The cells should be washed with PBS to make them free from serum and other unnecessary enzymes, which may interfere during the process. The magnetic field will be applied for 30 minutes to 8 hours depending the cell kinds and experience in incubator. After that media can be changed to serum media.

DNA needed per square centimeter space is around 25 to 250 ng. This can help to calculate the total requirement for experiments.

Magnetic bead antibody isolation kit

Cell Isolation

Exosome

Genekam cells catcher

Others

These products are developed and manufactured by Genekam Biotechnology AG in Germany.
* All prices are ex-factory, Duisburg, Germany and the products are only for research use